Tissue Engineering Micodevice

Tissue Engineering Micodevice

Student(s):

Leah Vandiver
Biomedical Engineering


Jessica Stukel´╗┐
Biomedical Engineering

Abstract

Microdevices allow for detection of an analyte on a small scale. This proposal is for an oxygen sensor with an embedded oxygen sensitive and reference fluorophore to determine the change in oxygen concentration. The advantages of this device are reduced time per experiment, consume less materials, easy viewing on a microscope, and reduced variability in the results. The overall function of the device is to use reporter particles to determine the oxygen concentration throughout the sensor channel. First, the oxygen sensitive and reference fluorophore are encapsulated in particles. These are evenly dispersed in the device to compare the change in oxygen concentration to a calibration point. Second, ports for oxygen rich and oxygen depleted media are used to establish an oxygen gradient which will be compared to computer simulations. The channel will be able to accommodate cells to observe cellular response to various oxygen concentrations. Necessary skills include fluorescent microscopy, cell culture, biomedical engineering lab experience, and expertise of fluid transport. A team of two BMEs would be appropriate.