Muscle Obtaining Bioreactor: Muscular Tissue Bioreactor

Muscle Obtaining Bioreactor: Muscular Tissue Bioreactor


Daniel Schnettgoecke
Mahsa Tourgoli
Nicholas Uffelmann
Eric Yeh



Dr. David W. Barnett

The goal of this design project was to construct a bioreactor specifically tailored to produce viable muscle tissue. This type of device increases the accessibility and availability of compatible tissues to recipients that have an area of tissue that is removed or diseased. To meet this goal, the design must provide both a biocompatible environment and an appropriate mechanical stimulation system. In this design, the bioreactor will contain a polymer scaffold on which the muscle cells will be grown over a two month incubation period. The bioreactor will be able to mechanically stimulate the growing tissue by cyclically stretching the scaffold. This stimulation serves to induce proper cell alignment which ensures that the tissue will exhibit properties similar to that of actual muscle. We were able to create this stimulation using an electric motor that rotated a cam lobe which caused cyclic displacement of the clips that secured the scaffold. This stimulation occurred at a rate of 17 RPM and a total displacement of 1.9 mm. The environment in which the scaffold is placed is also critical for healthy tissue development. Hence, this device allows for pH regulation and proper nutrient mixing. In addition, a sterilization test verified that the chamber of the bioreactor was free from microbial contamination. The device created by this design needs to be tested further and may need minor modifications before it is ready to be used to grow tissues. When this device actually does produce a tissue specimen, it will important to examine the cellular structure and mechanical properties of the resulting specimen to ensure that the mechanical stimulation system of the bioreactor induces proper cell alignment.